| |
|
|
| Registros recuperados : 24 | |
| 1. |  | NICIURA, S. C. M.; PERECIN, F.; SARAIVA, N. Z. Controle epigenético do desenvolvimento e do envelhecimento em mamíferos. In: NICIURA, S. C. M.; SARAIVA, N. Z. (Ed.). Epigenética: bases moleculares, efeitos na fisiologia e na patologia, e implicações para a produção animal e a vegetal. Brasília, DF: Embrapa, 2014. Cap. 7, p. 155-187.| Biblioteca(s): Embrapa Amazônia Oriental. |
|    |
| 3. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; GARCIA, J. M. Demecolcine effects on microtubule kinetics and chemically assisted enucleation of bovine oocytes. Cloning and Stem Cells, v. 11, n. 1, p. 141-152, mar. 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 4. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; VANTINI, R.; GARCIA, J. M. Efeitos da demecolcina sobre a cinética da maturação nuclear e a migração dos grânulos corticais em oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1276, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007. p. 1276.| Biblioteca(s): Embrapa Pecuária Sudeste. |
|  |
| 5. | | MÉO, S. C.; YAMAZAKI, W.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; LEAL, C. L. V.; GARCIA, J. M. Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments. Zygote, v. 15, p. 295-306, nov. 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 6. | | DEL COLLADO, M.; SARAIVA, N. Z.; LOPES, F. L.; CRUZ, M. H.; GASPAR, R. C.; OLIVEIRA, C. S.; PERECIN, F.; GARCIA, J. M. Efeitos da redução ou substituição do soro fetal bovino por outros compostos na maturação in vitro de oócitos bovinos. Pesquisa Veterinária Brasileira v. 34, n. 7, p. 689-694, jul. 2014.| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
| |
| 7. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; NICIURA, S. C. M.; FERREIRA, C. R.; OLIVEIRA, C. S.; GARCIA, J. M. The effects of ovalbumin as a protein source during the in vitro production of bovine embryos. Revista Brasileira de Zootecnia, v. 40, n. 10, p. 2135-2141, oct. 2011.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 8. | | SARAIVA, N. Z.; PERECIN, F.; MÉO, S. C.; FERREIRA, C. R.; TETZNER, T. A. D.; OLIVEIRA, J. C.; GARCIA, J. M. Effects of demecolcine on microtubule composition and chemically assisted enucleation of bovine oocytes. Reproduction, Fertility and Development, v. 20, n. 1, p. 107-108, 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 9. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; OLIVEIRA, C. S.; MONTEIRO, F. M.; LIMA, M. R.; NICIURA, S. C. M.; FERREIRA, C. R.; GARCIA, J. M. Effects of embryonic fluid and serum replacer as protein sources for in vitro maturation of bovine oocytes. In: INTERNATIONAL SYMPOSIUM ANIMAL BIOLOGY OF REPRODUCTIVE, 3., 2010, Águas de São Pedro: CBRA: USP/FMVZ, 2010| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 10. | | LANDIM JR, L. P.; FERREIRA, C. R.; PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; MIGLINO, M. A.; GARCIA, J. M. Análise da expressao gênica do VEGF (Vascular Endothelial Growth Factor) em placentas bovinas de animais clonados, produzidos in vitro e in vivo, pelo método de RT-PCR semiquantitativo. ARS VETERINARIA, v. 22, n. 3, p. 217-222, 2006.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 11. | | NICIURA, S. C. M.; FERREIRA, C. R.; PERECIN, F.; YAMAZAKI, W.; LEAL, C. L. M.; GARCIA, J. M.; MEIRELLES, F. V. Desenvolvimento embrionário, visualização de pronúcleos e transferência pronuclear após centrifugação de zigotos bovinos em meio com citocalasina. São Carlos, SP: Embrapa Pecuária Sudeste, 2007 34 p. (Embrapa Pecuária Sudeste, Boletim de pesquisa e desenvolvimento,11). ISSN 1981-2078| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 12. | | MEO, S. C.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; TETZNER, T. A. D.; YAMAZAKI, W.; LEA, C. L. V.; MEIRELLES, F. V.; GARCIA, J. M. Karyoplast exchange between strontium- and 6-DMAP-parthenogenetically activated zygotes of cattle. Animal Reproduction Science, v. 116, n. 3-4, p. 381-385, dec. 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 13. | | FERREIRA, C. R.; MEIRELLES, F. V.; YAMAZAKI, W.; CHIARATTI, M. R.; NICIURA, S. C. M.; PERECIN, F.; SMITH, L. C.; GARCIA, J. M. The kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos. Cloning and Stem Cells. v. 9, n. 4, p. 618-629, dec. 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 14. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in in vivo- and in vitro-produced bovine embryos and chorio-allantoic membranes. Genetics and Molecular Research, v. 8, n. 1, p. 76-85, 2009.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 15. | | PERECIN, F.; NICIURA, S. C. M.; YAMAZAKI, W.; FERREIRA, C. R.; BIASE, F. H.; MERIGHE, G. K. F.; MEIRELLES, F. V.; GARCIA, J. M. Imprinted gene expression in vivo-and in vitro-produced bovine fetuses and placentas. Reproduction, Fertility and Development, v. 20, n. 1, p. 173, 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 16. | | FERREIRA, C. R.; BURGSTALLER, J. P.; PERECIN, F.; GARCIA, J. M.; CHIARATTI, M. R.; MÉO, S. C.; MULLER, M.; SMITH, L. C.; MEIRELLES, F. V.; STEINBORN, R. Pronounced segregation of donor mitochondria introduced by bovine Ooplasmic tranfer to the female germ-line. Biology of Reproduction, v. 82, n. 03, p. 563-571, mar. 2010.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 17. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERREIRA, C. R.; MÉO, S. C.; OLIVEIRA, C. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Efeitos da substituição do soro fetal bovino (SFB) e da albumina sérica bovina (BSA) pela ovalbumina (OVA) na produção in vitro de embriões bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1181, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 18. | | PERECIN, F.; YAMAZAKI, W.; FERREIRA, C. R.; MÉO, S. C.; BIASE, F. H.; MERIGHE, G. K. F.; SARAIVA, N. Z.; TETZNER, T. A. D.; MEIRELLES, F. V.; GARCIA, J. M. Expressão de DNA metiltransferases em blastocistos bovinos produzidos in vivo e in vitro. Acta Scientiae Veterinariae, v. 35, supl. 3, p. 1181, 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 19. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERREIRA, C. R.; MÉO, S. C.; OLIVEIRA, C. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Avaliação da influência da ovalbumina (OVA) como suplemento protéico durante a etapa de fecundação in vitro de oócitos bovinos. Acta Scientiae Veterinariae, v. 35, supl. 3, p. s1181, 2007; In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 21., 2007, Salvador. Anais... Salvador: UFRGS, 2007.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| 20. | | TETZNER, T. A. D.; SARAIVA, N. Z.; PERECIN, F.; FERRERIA, C. R.; NICIURA, S. C. M.; OLIVEIRA, C. MELO, D. S.; MELO, D. S.; MONTEIRO, F. M.; VANTINI, R.; GARCIA, J. M. Avaliação da qualidade em embriões PIV com a substituição do soro fetal bovino e da albumina sérica bovina pela ovalbumina. Acta Scientiae Veterinariae, v. 36, (Supl. 2), 2008.| Biblioteca(s): Embrapa Pecuária Sudeste. |
| |
| Registros recuperados : 24 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
|
Data corrente: |
02/01/2008 |
|
Data da última atualização: |
08/03/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
Internacional - A |
|
Autoria: |
FERREIRA, C. R.; MEIRELLES, F. V.; YAMAZAKI, W.; CHIARATTI, M. R.; NICIURA, S. C. M.; PERECIN, F.; SMITH, L. C.; GARCIA, J. M. |
|
Afiliação: |
CHRISTINA RAMIRES FERREIRA, UNESP; FLÁVIO VIEIRA MEIRELLES, USP; WALT YAMAZAKI, UNESP; MARCOS ROBERTO CHIARATTI, USP; SIMONE CRISTINA MEO NICIURA, CPPSE; FELIPE PERECIN, UNESP; LAWRENCE CHARLES SMITH, UNIVERSITÉ DE MONTRÉAL; JOAQUIM MANSANO GARCIA, UNESP. |
|
Título: |
The kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
Cloning and Stem Cells. v. 9, n. 4, p. 618-629, dec. 2007. |
|
DOI: |
10.1089/clo.2006.0082 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either undergoes replication or escapes degradation during cleavage, although it is degraded later after the blastocyst stage or lost during somatic development, as revealed by the lack of donor cell mtDNA at birth. MenosThe mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either un... Mostrar Tudo |
|
Palavras-Chave: |
Bovine embryos; Cell mtDNA; Embryonic. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 02452naa a2200253 a 4500
001 1048156
005 2023-03-08
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1089/clo.2006.0082$2DOI
100 1 $aFERREIRA, C. R.
245 $aThe kinetics of donor cell mtDNA in embryonic and somatic donor cell-derived bovine embryos.$h[electronic resource]
260 $c2007
520 $aThe mechanisms controlling the outcome of donor cell-derived mitochondrial DNA (mtDNA) in cloned animals remain largely unknown. This research was designed to investigate the kinetics of somatic and embryonic mtDNA in reconstructed bovine embryos during preimplantation development, as well as in cloned animals. The experiment involved two different procedures of embryo reconstruction and their evaluation at five distinct phases of embryo development to measure the proportion of donor cell mtDNA (Bos indicus), as well as the segregation of this mtDNA during cleavage. The ratio of donor cell (B. indicus) to host oocyte (B. taurus) mtDNA (heteroplasmy) from blastomere(NT-B) and fibroblast(NT-F) reconstructed embryos was estimated using an allele-specific PCR with fluorochrome-stained specific primers in each sampled blastomere, in whole blastocysts, and in the tissues of a fibroblast derived newborn clone. NT-B zygotes and blastocysts show similar levels of heteroplasmy (11.0% and 14.0%, respectively), despite a significant decrease at the 916 cell stage (5.8%; p < 0.05). Heteroplasmy levels in NT-F reconstructed zygotes, however, increased from an initial low level (4.7%), to 12.9% (p < 0.05) at the 9?16 cell stage. The NT-F blastocysts contained low levels of heteroplasmy (2.2%) and no somatic-derived mtDNA was detected in the gametes or the tissues of the newborn calf cloned. These results suggest that, in contrast to the mtDNA of blastomeres, that of somatic cells either undergoes replication or escapes degradation during cleavage, although it is degraded later after the blastocyst stage or lost during somatic development, as revealed by the lack of donor cell mtDNA at birth.
653 $aBovine embryos
653 $aCell mtDNA
653 $aEmbryonic
700 1 $aMEIRELLES, F. V.
700 1 $aYAMAZAKI, W.
700 1 $aCHIARATTI, M. R.
700 1 $aNICIURA, S. C. M.
700 1 $aPERECIN, F.
700 1 $aSMITH, L. C.
700 1 $aGARCIA, J. M.
773 $tCloning and Stem Cells.$gv. 9, n. 4, p. 618-629, dec. 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
